The rigorous screening process for stool donors adhered to the guidelines outlined in the Chinese Fecal Microbiota Transplantation Donor Guidelines [31, 32] and performed by Xiamen Treatgut Biotechnology Co., Ltd, China. Thorough scrutiny of the donor’s medical history and lifestyle practices was undertaken to preclude any potential exposure to infectious agents or engagement in substance abuse.
Comprehensive evaluations, including physical examinations and blood analyses, were conducted to eliminate the presence of gastrointestinal, metabolic, or neurological disorders. Parameters assessed during the blood examination encompassed complete blood count, blood glucose levels, electrolyte concentrations, and inflammatory markers. Additionally, liver function tests and thyroid function tests were administered. Serological screening tests were performed to ascertain the donor’s status with respect to human immunodeficiency virus (HIV), syphilis, and hepatitis A, B, and C. Furthermore, meticulous examinations were conducted to test the presence of pathogenic bacteria such as Shigella spp., Salmonella spp., Campylobacter spp., Yersinia spp., and toxin-producing C. difficile[31]. Screening for rotavirus, fecal ova, and parasites was also conducted.
The identified donor, four current college students (two females and two males), yielded negative results across all administered tests and examinations. The donor exhibited a non-smoking habit, maintained a state of good health, did not ingest any medications, and possessed a body mass index (BMI) within the range of 18.5–23.9 kg/m². Importantly, the donor exhibited no familial relationships to any participants enrolled in this study.
