cryoprotectants

  1. Michael Harrop

    Other Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses (Jul 2024, n=3) "changes induced in fecal microbiota with different storage conditions are marginal compared to the variations between horses"

    https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-024-04166-w FYI, adding saline makes freezing damage worse. It would be interesting to see these studies test pure stool without anything added. Or maybe they did? I'm not sure what they mean by that. I think they did though:
  2. S

    Enhancing Antifreeze in stool/FMT capsules, glycerol vs maltodextrin, -80 (medical freezer) vs -20 (home freezer)

    I was reading through the published information on how OpenBiome processes their stool samples into FMT preparations. They state that the stool is dissolved in a glycerol/saline solution, consisting of 12.5% glycerol in 0.9% NaCl, before storing in a -80C freezer...
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