Healthy wife as donor? GI-MAP shows bacterial imbalances Donors 

mctopher

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Hi,


I’m considering using my wife’s stool as my FMT donor. She is healthy, 28 years old, and I would have daily access to fresh samples. She is much calmer than I am, sleeps well, and exercises regularly.


I went through the standard donor screening questions with her, and everything looked good.


We both recently completed GI-MAP tests through Nordic Labs (we are in Europe). Her results show a fair number of bacterial imbalances, even though she is clinically healthy and symptom-free.


Would it still make sense for me to use her as a donor? I couldn’t find any information on the website about how to interpret GI-MAP results specifically for donor selection.


I’ve attached both of our stool reports for reference.


Thank you for any input!
 

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  • GI-MAP_Wife_2026-07-20_ANON_readable.pdf
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I looked over them and it seems all in all compared to other GI-MAP results I've seen it's not that bad.

She has a lot of staph but so do you, so it's likely a shared environment thing that you probably won't risk too much getting worse from. The one thing I did notice was that she has quite high Pseudomonas, and a lot of it is aeruginosa. There are quite some people who have that, both active HM donors did as well. It seems tentatively that female donors MAY be more likely to have higher Pseudomonas and that might not always be bad--I could definitely see there even being some strains of Pseudomonas with beneficial, probiotic-like properties, given their flexible metabolism and robust respiratory capabilities, and most species aren't really all that well studied. However aeruginosa is well studied and is known to be a particularly pro-inflammatory and invasive species that you almost certainly don't want much of in your gut or most other places (on intact skin might be OK).

There was once a participant in a FMT group who had a personal donor who he had previously gotten quite good results from. Then at one point the donor's stool appearance changed along with high amounts of Pseudomonas showing up on her stool tests and from then on this donor gave him (the FMT recipient) IBS-like symptoms when using her stool in addition to more modest benefits.

In light of this it was interesting to see that she has high anti-gliadin antibodies. That suggests she might be pre-celiac, which is definitely something to be cautious about (I don't mean just in regards to possible FMT, I mean in regards to ensuring her future health in general). Unfortunately I don't know how much is known about lifestyle changes one can do to try and prevent progression from "pre-celiac" to actual celiac disease but I'd imagine that rotating diet would be helpful, as it's known to be helpful for food tolerance in general. I.e. don't have gluten with every meal and rotate in some non-gluten starches and grains. Also I'd suggest she not overconsume things like beer that combine gluten with gut irritants such as alcohol, if she has a lot of that maybe replace some with wine. Don't try to quit gluten "cold-turkey" as complete avoidance of any food if anything probably encourages loss of tolerance. Again, none of this is proven to prevent the disease by any means but a lot of this is common sense extrapolating from what people have noticed with other food intolerances.

I'm focusing on this because if she were to become intolerant to gluten, it would make life quite hard--I've avoided gluten at times in the past when I seemed to be sensitive and know firsthand what an incredible pain it is. Why I said at the beginning that it was "interesting" was that there's a theory out there that the autoimmune response involved in celiac may start as an immune reaction to Pseudomonas species, since some of them have peptides of similar sequence to wheat peptides. It's far from generally accepted but it's a theory.

But overall, as I said I've seen much "worse" GI-MAP results, and much of the overgrowths seem shared between you so there's relatively little (aside from the Pseudomonas) that you'd be introducing freshly to your system.
 
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Hi
We went through the „standard questions“ here on this wiki:
https://humanmicrobiome.info/fmt-questionnaire/

My surprise was after positive answers that she has this many imbalances and curious if it would still be helpful for me to use her stool.

Thank you SFBayFMT5 for the detailed answer. Is your conclusion makes sense for me then to use her stool? From what you wrote, you think it would still be beneficial, right? And for her to pay attention to gluten consumption.
And I find it interesting That, although she is very health, she still has these im balances.
 
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Does that mean its safe for him or pointless? I mean, not everyone is looking for a superdonor like Michael, so if that is safe is enough for OP, it may be worth it.
Ignore it. It's not a valid conclusion whatsoever. That is not a valid use of the GI-MAP.

My surprise was after positive answers that she has this many imbalances and curious if it would still be helpful for me to use her stool.
You clearly haven't reviewed the wiki. Stop posting threads & comments till you do. Please read the rules as well: https://forum.humanmicrobiome.info/threads/rules-info-submission-guidelines-features.20/
 
You clearly haven't reviewed the wiki
@mctopher Michael won’t explain so I will:

https://humanmicrobiome.info/fmt/#screening

Your wife has pre-dysbiosis gut, like most people. It doesn’t mean that it is healthy, it just means it’s not severe enough to cause problems. That means that it will either have no major effect or it would be detrimental if you have FMT from her. I asked about safety but in hindsight, it’s not guaranteed and that is Michael’s biggest motivation for the superdonor hunt.

This is why FMT is a gamble in its current state, and taking the steps to find an real healthy person takes major research effort without government and institutional support, like in Michael’s case.
 
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If you're basing it off the tests alone, I would argue there to be no real benefit from taking your wife's stool. In fact, I would argue your gut has a higher chance of balancing itself out from diet alone than your wife's gut, simply because it has higher amounts of the ecosystem-balancing microbes.

The questionnaire linked to on this website is better than the questionnaires used by others. Some of the questions on there require you to explain things in detail. Therefore, what is "good enough" for you does not necessarily mean it is good enough for those of us who understand the reasoning behind the questionnaire.

If sleeping better and less stressed or anxious is your only complaint, FMT should not be on your mind.
 
If you're basing it off the tests alone, I would argue there to be no real benefit from taking your wife's stool. In fact, I would argue your gut has a higher chance of balancing itself out from diet alone than your wife's gut, simply because it has higher amounts of the ecosystem-balancing microbes.
In my experience, diet alone almost never fixes anything on its own. It only is a stopgap until you can fill in the gaps in your microbiome from a donor. Now, it's certainly possible to acquire bad dietary habits from dealing for years with an out-of-whack gut, that need to be corrected once the microbiome is restored to keep from perpetuating problems once the gut is fixed. For instance, at some point I started eating way too much protein for my gut and it caused problems that only now are starting to get fixed, since my microbiome got better to the point where the excessive protein intake got "unmasked" as the cause of these problems once others were solved.

I don't believe that anyone knows what microbes are "ecosystem-balancing" well enough to say that someone has "more ecosystem-balancing microbes" than another person.

The questionnaire linked to on this website is better than the questionnaires used by others. Some of the questions on there require you to explain things in detail. Therefore, what is "good enough" for you does not necessarily mean it is good enough for those of us who understand the reasoning behind the questionnaire.

If sleeping better and less stressed or anxious is your only complaint, FMT should not be on your mind.

Actually, there have been many times when neuropsychiatric issues, including severe insomnia, depersonalization, etc. were the most acute sign of a dysregulated microbiome, and the ones to most dramatically improve post-FMT. I did also have food reactions, diarrhea, or more lately gut pain, though the pain started very late in the game and before my first "miracle" FMT it was completely absent.

It's also important to clarify that there are two quite different definitions of "safety". One of them, which is the one used by most FMT researchers, is stool not having acute pathogens that might put you in the hospital or even kill you. Things like C. diff, cholera, HIV, hepatitis B, etc. Your wife's stool, according to the GI-MAP, doesn't have these. The kind of overgrowths she has (and you have) are not ones that an ordinary doctor, one who isn't specifically focused on SIBO, would see it medically necessary to treat immediately by any means necessary, at least not in the gut (Pseudomonas is that kind of bug when it's in the lungs or the blood, for instance, and Staph aureus is when it's in an abscess). This kind of safety is not that hard to find.

The OTHER definition of "safety" is "won't make your dysbiosis, and whatever condition you have that's caused by it, worse". This kind of safety is much more difficult to find, and Michael knows of cases where even donors at a one-in-a-million level of health by all criteria he knows about still made recipients worse. So nobody can prove beyond a doubt that any donor is "safe" in this second sense, and several donors I have tried were "unsafe" in this regard. However, in my personal experience, the damage from this kind of donor can be corrected by using a better donor at a later time.

Also another thing worth mentioning, given my experience with oral transplants so far... it might be that you're surprised with how little using your wife's stool would do given that you're already presumably quite intimate and sharing microbes other ways (not to mention also sharing living space and diet). It's certainly not impossible, there are husband/wife pairs where one cured the other's bipolar for instance using stool, that obviously didn't get accomplished otherwise. But if you were to try your wife and basically nothing happened, it doesn't mean that using a stranger with all other methods the same that you wouldn't get a benefit.
 
Also I should add that at least at this point in time, the first kind of "safety" has much more to do with lab tests than the second. With regards to the second, it's more like your doing research, where you're helping gather data on what a GI-MAP result of a "safe" or "unsafe" donor looks like from your own experience, as opposed to being able to tell definitively from the test whether the donor is or isn't "safe". In the future there may well come a time when we CAN tell the safety of donors in the second test with lab testing--however a questionnaire would still be helpful as an initial filter to determine whom to test. However in the present, the questionnaire is actually significantly more helpful than a test on the stool itself, as regards the second definition. Good health habits as assessed by the questionnaire can also reduce the likelihood of being unsafe in the first sense, but there lab testing will almost certainly always be more accurate.
 
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